Vancouver , BC , Canada Background Activity of Reverse Transcriptases

نویسندگان

  • Kimberly J. Reid
  • Colleen C. Nelson
چکیده

images were scanned using a Hewlett Packard 6300 dpi resolution scanner. The MeP footprint of androgen receptor cooperatively bound to DNA revealed for the first time guanines that became hypersensitive to DMS methylation (Figure 1). Since DMS normally methylates guanines uniformly, the hypermethylation of guanine indicates local distortion to the DNA. Therefore, MeP is an ideal footprinting method for detecting local distortion to the DNA that result from allosteric interactions between a transcription factor and the DNA. This method greatly improves the utility of methylation protection as a tool for readily examining DNA-protein interactions.

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تاریخ انتشار 2001